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KMID : 0379120130410030160
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Korean Journal of Mycology
2013 Volume.41 No. 3 p.160 ~ p.166
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Optimal Extraction and Characteristics of Lignocellulytic Enzymes from Various Spent Mushroom Composts
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Lim Seon-Hwa
Lee Yun-Hae
Kang Hee-Wan
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Abstract
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Recovery of α-amylase (EC 3.2.1.1), lignin peroxidase (EC 1.11.1.14), laccase (EC 1.10.3.2), xylanase (EC 3.2.1.8), β
xylosidase (EC 3.2.1.37), β-glucosidase (EC 3.2.1.21) and cellulase (EC 3.2.1.4) from spent mushroom composts (SMCs) of Pleurotus cornucopiae, Pleurotus ostreatus, Pleurotus eryngii, Hericium erinaceum, Lyophyllum ulmarium, Agrocybe cylindracea, Lentinus lepideus, and Flammulina velvtipes were investigated using different extraction buffers. The maximum recovery of the enzymes was mostly detected in SMC extracts with tap water and 0.25% Triton X-100 by shaking incubation (200 rpm) for 2 h at 4o C. The xylanase (152 U/g) and laccase (8.1 U/g) activities were the highest in SMC extracts from F. velvtipes and P. eryngii. In addition, high enzymatic activities of α-amylase (3.6 U/g) and cellulase (3.4 U/g) was detected in SMC extract of A. cylindracea. Futhermore, cellulase and laccase activities of SMCE from P. eryngii were compared to commercial enzymes.
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KEYWORD
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Lignocellulytic enzymes, Recovery, Spent mushroom composts
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